8/15/2023 0 Comments Per2 snapgeneAlthough these therapy techniques have been greatly improved, the 5-year survival rate of patients with OSCC has remained at approximately 50% over the last three decades and has not been dramatically improved. Currently, the conventional treatment of OSCC is surgical treatment, adjuvant radiotherapy and chemotherapy. OSCC (OSCC: oral squamous cell carcinoma) is the most common malignant tumor of the head and neck. Keywords: clock genes, oral cancer, autophagy, carcinogenesis, Period 2 Introduction Per2 could potentially be used as a valuable therapeutic marker for OSCC. In conclusion, our research results demonstrate that Per2 suppresses OSCC progression by motivating autophagy, as well as inhibiting cell proliferation and promoting apoptosis, which were mediated by autophagy, in a PI3K/AKT/mTOR pathway-dependent manner. An in vivo tumorigenesis assay also confirmed that overexpression of Per2 suppresses the growth of OSCC. Furthermore, when autophinib, an autophagy inhibitor, was added to Per2-overexpressing OSCC cells, the decreased proliferation and increased apoptosis were significantly restored. When the AKT activator SC79 was added to Per2-overexpressing OSCC cells, the increased autophagy, apoptosis and decreased proliferation were significantly rescued. In contrast, in Per2-silenced OSCC cells, the expression levels of PIK3CA, p-AKT, p-mTOR, p62 and Beclin1 were significantly enhanced and the LC3B II/I ratio was significantly reduced. In Per2-overexpressing OSCC cells, the expression levels of PIK3CA, p-AKT, p-mTOR, p62 and Beclin1 were significantly reduced and the LC3B II/I ratio was significantly increased. The opposite results were obtained in Per2-silenced OSCC cells. Overexpression of Per2 promoted autophagy and apoptosis in OSCC cells and inhibited proliferation. We discovered that the expression of Per2 decreases in OSCC cells. In this study, OSCC cells with stable overexpression or silencing of Per2 were established to explore their biological functions and mechanism in vivo and in vitro. However, the biological functions and mechanism of Per2 in OSCC (OSCC: oral squamous cell carcinoma) remain unclear. The current studies reveal that the clock gene Per2 is expressed at lower levels in a variety of tumors and plays a significant tumor suppressor role. Select the file that you have just downloaded and select import option Reference Manager (RIS). Overexpression of the clock gene Per2 suppresses oral squamous cell carcinoma progression by activating autophagy via the PI3K/AKT/mTOR pathway.
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